# DotDr (dotdr.co.za)

> Specialized AI Model Orchestration for South African Pathology Networks, Genetics Laboratories & Research Biobanks.

## Overview
DotDr (Pty) Ltd is a South African biomedical artificial intelligence systems engineering studio led by Duncan Luke (Lead AI Systems Specialist). DotDr orchestrates frontier Google AI models—specifically **AlphaGenome**, **AlphaFold2 / ColabFold**, and **MedLM**—into high-throughput, sovereign, in-country clinical genomics pipelines.

## Key Capabilities & Core Workflows
1. **AlphaGenome Variant Triage:**
   - Evaluates non-coding Variants of Uncertain Significance (VUS) across 9+ billion genomic positions using AlphaGenome Variant Impact (AVI) scores.
   - Detects cryptic splice sites, promoter disruptions, and distal enhancer alterations.
2. **Structural Delta-Analysis (AlphaFold2 / ColabFold):**
   - Automates mutant vs. wild-type 3D structural superposition.
   - Calculates C-alpha RMSD, residue-level delta-pLDDT stability shifts, and Predicted Aligned Error (PAE) matrices to confirm or rule out pathogenic protein destabilization.
3. **Sovereign Clinical LLM Report Drafting:**
   - Generates ACMG-compliant draft clinical summary reports directly from structured VCF files and PubMed literature evidence.
   - Operates with human-in-the-loop review gates for registered South African pathologists and medical geneticists.

## Regulatory & Data Sovereignty
- **POPIA (Protection of Personal Information Act):** Section 19 security alignment. Zero Personally Identifiable Information (PII) is processed; pipelines ingest de-identified genomic coordinates only.
- **HPCSA (Health Professions Council of South Africa):** Booklet 2 & 10 compliant assistive decision support.
- **In-Country Cloud Residency:** Primary compute cluster hosted in Google Cloud Platform (GCP) `africa-south1` (Johannesburg) or on-premise behind institutional firewalls.

## Commercial Engagements & Pricing
- **Anonymized Pilot Trial:** R0 / 30-day trial evaluating 20–50 historical laboratory variants.
- **Custom AI Pipeline Sprint:** R160,000 ZAR fixed 2-week production integration (LIMS, VCF parsers, custom AI agents).
- **High-Throughput Compute:** Cost-plus pass-through GPU clusters in GCP Johannesburg.
- **Managed Support & SLA:** Model calibration, continuous database updates, uptime guarantees.

## Contact & Inquiry
- Website: https://dotdr.co.za
- Domains: dotdr.co.za, dotdr.ai, dotdr.co
- Engineering Briefings: Automated dispatch from duncan@dotdr.co.za